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Molecular determinants of ageing - quantitative response of Drosophila to adult-specific RNAi
| Project type: | Research Project |
| Programme: | Life Sciences |
| Call: | Life Sciences Call 2005 |
| Start: | 01.03.2006 |
| Duration: | 3,50 years |
| Grant awarded: | 582.400 € |
| Keywords: | Drosophila ageing research, RNA interference (RNAi), transcriptional expression profiling by microarrays, Independent Component Analysis, Bayesian inference, information fusion |
David Kreil
University of Natural Resources and Life Sciences, Vienna
Department of Biotechnology
| Project partners: | Nadège Minois (Research Institute for Molecular Pathology, Vienna) Barry Dickson (Research Institute for Molecular Pathology, Vienna) |
We investigate molecular mechanisms of ageing in complex multicellular organisms by adult-specific gene silencing in Drosophila. Triggering temperature sensitive RNAi only in adult life allows, for the first time, the complete separation of developmental effects in a screen for longevity. In addition, multi-dimensional phenotypic and behavioural assays over the entire adult life of the flies permit a focus on lines that live long and well. Molecular effects of candidate genes on ageing are thoroughly studied over time and genome-wide using RT-PCR and microarray time-courses. Integrated analysis of molecular, phenotypic, and behavioural data requires development of modern probabilistic methods at all stages of the project, providing 1) optimal experimental design, 2) identification of causal consequences of target gene silencing, and 3) sensitive detection of molecular processes differentially affected during ageing.
